Reconstitution of Iron Oxidase from Sulfur-Grown Acidithiobacillus ferrooxidans
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چکیده
منابع مشابه
Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.
The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.
متن کاملAnalysis of phospholipids in membrane of Acidithiobacillus ferrooxidans grown in liquid (ferrous sulphate and sodium thiosulphate) and solid (sulfur) substrate
Phospholipids (PLs) are the important components of cell membrane of all living organisms. The PL or the cell membrane of the bacteria are the least studied subject as it involves large quantities of material and few methods to assay. The novel study was designed to understand if the cell membrane remains same or it changes during different conditions. The bacteria, Acidithiobacillus ferrooxida...
متن کاملCytochromes c of Acidithiobacillus ferrooxidans.
The chemolithoautotrophic Gram-negative bacterium Acidithiobacillus ferrooxidans is versatile and can grow on a number of electron donors and acceptors. In the A. ferrooxidans ATCC 23270 genome, computer analysis identified 11 genes encoding putative cytochromes c. At least eight putative cytochromes c were differentiated on gels in ATCC 33020 cells grown on ferrous iron or sulfur. All these cy...
متن کاملThe ferric iron uptake regulator (Fur) from the extreme acidophile Acidithiobacillus ferrooxidans.
Acidithiobacillus ferrooxidans is a Gram-negative bacterium that lives at pH 2 in high concentrations of soluble ferrous and ferric iron, making it an interesting model for understanding the biological mechanisms of bacterial iron uptake and homeostasis in extremely acid conditions. A candidate fur(AF) (Ferric Uptake Regulator) gene was identified in the A. ferrooxidans ATCC 23270 genome. Fur(A...
متن کاملPurification and characterization of sulfide:quinone oxidoreductase from an acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans.
Sulfide:quinone oxidoreductase (SQR) was purified from membrane of acidophilic chemolithotrophic bacterium Acidithiobacillus ferrooxidans NASF-1 cells grown on sulfur medium. It was composed of a single polypeptide with an apparent molecular mass of 47 kDa. The apparent K(m) values for sulfide and ubiquinone were 42 and 14 muM respectively. The apparent optimum pH for the SQR activity was about...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00787-08